NCBI Organism:Mus musculus; Cell Types:CNS neuron (sensu Vertebrata) Cell Components:dendrite, axon;

Licensing

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40022^* Cite

Description

Z projection through a through-focus series of a medium spiny neuron of the neostriatum from a dopamine transporter knock out mouse, injected with Lucifer Yellow and then photoconverted. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database, where the reconstruction of the entire cell can also be found.

Technical Details

Tissue was prepared from a 6 month adult male mouse strain, C57BL/129SvJ. Tissue sections, 100µm, were generated using a vibratome. The entire through series of images were gathered using a Bio-Rad Radiance 2000 with a 40X 0.75 NA.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: CNS neuron (sensu Vertebrata)
Cellular Component: dendrite; axon

Attribution

Names: Diana Price
Link: CCDB 3693

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40022
Archival Resource Key (ARK): ark:/b7295/w9cil40022

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Visualization Methods: intracellular filling; lucifer yellow
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
X	512px	——