A 512 by 512 image from the slice by slice reconstruction of a caulobacter crescentus specimen. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
Caulobacter cultures (strain 3724) were prefixed with 1% acrolein and 2.5% glutaradehyde, followed by high pressure freezing, and freeze substitution using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate, and embedding in Durcupan. Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 kV.
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