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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40009*  Cite 

Computed slice through a tomographic reconstruction of the pre- and post-synaptic structures of the calycal synapse onto a chick ciliary ganglion neuron. This slice was derived from the 3rd of 6 serial volumes comprising the entire reconstruction. For more information, see: Shoop et al. Ultrastructure of a somatic spine mat for nicotinic signaling in neurons. J Neurosci. 2002 Feb 1;22(3):748-56. PMID: 11826104This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Embryonic day (E) 15 chicks or 2-year-old adult chickens were perfusion-fixed with 2% paraformaldehyde plus 2% glutaraldehyde in cacodylate buffer, pH 7.4. Ciliary ganglia were removed, transferred to fresh fixative, and incubated for 3 hr at room temperature. Adult ciliary ganglia were cut into 1 mm3 pieces. After being rinsed several times in 0.1 M sodium cacodylate buffer, pH 7.4, the tissue was treated for 30 min with 2% osmium tetroxide in 0.1 M sodium cacodylate and then counterstained with uranyl acetate. The ganglia were dehydrated in a series of ethanol solutions followed by two rinses in acetone, infiltrated with Durcupan ACM resin (Electron Microscopy Sciences, Fort Washington, PA), allowed to polymerize for 24 hr at 60° C, and then sectioned. For serial tomography a continuous series of 10 1-µm-thick sections was prepared to encompass a complete spine mat. For traditional thin section analysis 100-nm-thick sections were made.

Biological Sources
NCBI Organism Classification
Gallus gallus gallus
Cell Type
peripheral neuron
Cellular Component
Rick Shoop
Darwin Berg
Mark Ellisman
Naoko Yamada
Ed Esquenazi
CCDB 3629
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Visualization Methods
uranyl salt
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 512px ——
X 512px ——