Portion of a single computed slice through a tomographic reconstruction of the myocardium of the left ventricle of a normal mouse. For more information see: Hayashi, T., Martone, M. E., Yu, Z., Thor, A., Doi, M., Holst, M., Ellisman, M. H. and Hoshijima, M., Three-dimensional electron microscopy reveals new details of membrane systems for calcium signaling in the heart. J. Cell Science (2009) PMID: 19295127. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
Tissue was fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.15M sodium cacodylate, blocked into small pieces, post-fixed for 1-3 hours, washed 3x10min in 0.15M sodium cacodylate, then incubated in: 0.8% potassium ferrocyanide and 2% osmium tetroxide in 0.15M sodium cacodylate overnight at room temperature. Following another set of washes 2 hours-overnight in 0.15M sodium cacodylate tissue was stained en bloc with 1% uranyl acetate 30min, followed by a brief wash in DDH2O and standard dehydration and embedding. Images were gathered using a JEOL 4000, magnification not given, accelerating voltage, 400.0 kV.
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