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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39994*  Cite 
Description

A single slice from the slice-by-slice reconstructed volume of a Caulobacter Crescentus specimen. This particular slice is taken from Z slice # 142. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone following this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius. Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 Kv, with the objective aperture set at 2.

Biological Sources
NCBI Organism Classification
Caulobacter crescentus CB15
Attribution
Names
Lucy Shapiro
Harley McAdams
Link
CCDB 3566
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39994
Archival Resource Key (ARK)
ark:/b7295/w9cil39994
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
Processing History
recorded image
Data Qualifiers
processed data
Sample Preparation
Methods
osmium tetroxide fixed tissue
cryofixed tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——