Licensing
Description
A spiny dendrite from neostriatum of a dopamine transporter knockout mouse. The cell was imaged using photoconverted Lucifer yellow, and a maximum intensity project image was created. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.
Technical Details
Mouse strain B6;129-/Slc6a3 tm2Mca was used in this preparation. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -60 to 60° in 2° increments. Magnification, 3000X; accelerating voltage, 3.0 MeV.
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- CNS neuron (sensu Vertebrata)
- Cellular Component
- dendritic spine
- dendrite
Attribution
- Names
- Diana Price
- Aki Laakso
- Michele Cyr
- Maryann Martone
- Naoko Yamada
- Andrea Thor
- Monica Berlanga
- Link
- CCDB 3563
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- FBbi:00000622
- detection of electrons
- illumination by electrons
- Parameters Imaged
- electron density
- Visualization Methods
- intracellular filling
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- formaldehyde fixed tissue
- glutaraldehyde fixed tissue
- tissue in epoxy resin embedment
- osmium tetroxide fixed tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | —— |
| Y | 512px | —— |