Single transmitted light section from a through focus series through a photoconverted medium spiny cell from the neostriatum of a wild type mouse. Flocculent material surrounding the labeled cell is likely photoconverted mitochondria or non-specific debris produced by the photoconversion procedure. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database, where the reconstruction of the entire cell can also be found.
Tissue was prepared from a 7 month adult male mouse strain, C57BL/129SvJ. Tissue sections, 100µm, were generated using a vibratome. The entire through series of images were gathered using a Bio-Rad Radiance 2000 with a 40X 0.75 NA.