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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39796*  Cite 

Basal body formation occurs most prominently in the equatorial region of the predivision cell. A new basal body begins to develop perpendicular to the mature basal body at its proximal anterior surface. The basal body lengthens and tilts toward the surface. At first no new accessory microtubular ribbons or kinetodesmal fibers are observed, only those accessories associated with the mature basal bodies are present. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg.19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Tetrahymena pyriformis
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
microtubule basal body
microtubule associated complex
Biological Context
Biological Process
microtubule basal body duplication
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 5008px 0.78nm
Y 6255px 0.78nm