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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39786*  Cite 
Description

Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] at the gastrula stage. Embryo was split open to reveal a nice cross-section through the outer epithelial layer and the blastocoel cavity. The fertilization envelope dissolves during swimming blastula stage; therefore, cilia and microvilli can be seen at the apical end of the epithelial layer. A few migrating primary mesenchyme cells can be seen within the blastocoel matrix. This is a lower magnification of CIL:39790. This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.

Technical Details

Images taken on a JEOL 35C SEM. Processing protocol: 1. 2% GTA in 75% MBL SW or FilteredSW FSW - fix for 1.5hrs. RT. 2. Rinse with 0.1M NaCacodylate buffer pH 7.4 3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold 4. Rinse in 0.1M NaCacodylate buffer ph 7.4 5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each 6. 100% - 3 changes - 15' each 7. Critical Point Dry 8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point). 9. Sputter coat with AuPd.

Biological Context
Biological Process
embryonic morphogenesis
cell migration
Attribution
Names
Louisa Howard
Link
Dartmouth EM Facility
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39786
Archival Resource Key (ARK)
ark:/b7295/w9cil39786
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
ethanol dehydration
critical_point dried specimen
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 374px ——