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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39727*  Cite 
Description

Transmission electron micrograph of mouse cardiac muscle (ventricle). The image illustrates the myofibrils as well as a sarcomere in the area of fibrils between the two Z-lines. Z-lines link actin filaments of one sarcomere to the next, while the sarcomere is responsible for cardiac muscle contractions.

Technical Details

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
cardiac muscle cell
Cellular Component
sarcomere
myofibril
Z disc
Biological Context
Biological Process
regulation of cardiac muscle contraction
Attribution
Names
Dee Lauzon
Sue Lancelle
Marian Rice
OTHER
Mount Holyoke College
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39727
Archival Resource Key (ARK)
ark:/b7295/w9cil39727
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
formaldehyde fixed tissue
ethanol dehydration
tissue in epoxy resin embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1800px ——
Y 1602px ——