The acidosome-like vesicle has a bull’s eye appearance with the invagination appearing in the middle of the vesicle. Other vesicles contain multiple layers resembling multilipid layers. Two vesicles with thick membranes and flocculent material may be lysosomes. Membranes of lysosomes of other cells tend to be thicker and have a polysaccharide luminal coat and do not have invaginations. TEM taken on 7/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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