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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39708*  Cite 

Transmission electron micrograph of an unbudded yeast cell treated with 60µM sertraline. Two autophagosomes containing membranous material are visible. Higher magnification images of ultrastructural features are available as CIL:39709, 39710, 40305, and 40306.

Technical Details

BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Type
Cellular Component
autophagic vacuole
autophagic vacuole lumen
autophagic vacuole membrane
Biological Context
Biological Process
sertraline treatment
organelle organization
Ethan Perlstein
Daniel Korostyshevsky
Princeton EM Core Facility
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 2160px ——
Y 2212px ——