Transmission electron micrograph of a budding yeast cell. Electron-dense (black) vacuoles that contain membrane-bound inclusions are visible. The image contributor asks Library users to post comments answering the following questions: What is the tubular organelle protruding into the bud? What about the structure that appears to be an autophagosome encapsulating organellar membrane material located center-right?
BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.
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