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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39686^* Cite

Description

Intestinal epithelium from the adult midgut of Drosophila melanogaster were immuno-stained for the cell periphery (integrin, red), nuclei (DAPI, blue) and stem cells (green). Nuclei are not seen in the tiny stem cells. The system was used to determine the role of intestinal stem cells in establishing the size of the gut in response to changes in food availability.

Technical Details

Midguts were fixed with 8% formaldehyde for 20 min at room temperature, and immunolabeled with primary antibodies bPS integrin (cf.6g11) and disc large (4F3), and stained with DAPI. The tissue was examined using a Leica TCs confocal microscope with a 63x N.A. 1.4 objective lens. Images were contrast enhanced and stem cells pseudocolored. See also: L E O'Brien et al., (2011) Altered modes of stem cell division drive adaptive intestinal growth. Cell 147:603-614.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: intestinal epithelial cell; stem cell
Cellular Component: nucleus; cell membrane

Biological Context

Biological Process

Attribution

Names: Lucy O'Brien; David Bilder
Link: NIGMS Biomedical Beat

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39686
Archival Resource Key (ARK): ark:/b7295/w9cil39686

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 555
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	400px	——
Y	307px	——