Licensing
Description
10-hour timelapse of Wild-type Saccharomyces cerevisiae growing in 2% raffinose. These experiments are the control experiments used for comparison to the effects of the double mutant Clb2dbΔ, Clb5Δ on the cell cycle control system in budding yeast. The images from two experiments done with Wild-type Saccharomyces cerevisiae are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbΔ, Clb5Δ are available as CIL:39626-39645 and CIL:39666-39685.
Technical Details
Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.
Biological Sources
- NCBI Organism Classification
- Saccharomyces cerevisiae
- (Baker's Yeast, Brewer's Yeast)
- Cellular Component
- cell surface
Biological Context
- Biological Process
- cell cycle checkpoint
- cell budding
- regulation of cell budding
Attribution
- Names
- David Ball
- Jean Peccoud
- Published
- Cell Cycle 10:6, 999-1009; March 15, 2011;
Grouping
This image is representative of a group of similar images.Imaging
- Image Type
- recorded image
- Imaging Mode
- phase contrast microscopy
- Parameters Imaged
- elastic scattering of photons
- Source of Contrast
- differences in amount of elastic light scattering
- Visualization Methods
- visualization of contiguous regions
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | 0.213μm |
| Y | 512px | 0.213μm |
| Time | 240 sec | 10 hr |