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Description
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Clb2. Clb 2 is a cyclin-dependent protein kinase regulator, involving in G2/M transition of mitotic cell cycle and positively regulating spindle pole body separation. These phase and GFP z-series images are part of an image group containing CIL 39556-39560. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.
Technical Details
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.
Biological Sources
- NCBI Organism Classification
- Saccharomyces cerevisiae
- (Baker's Yeast, Brewer's Yeast)
- Cellular Component
- CLB2 protein
- spindle pole body
- spindle
- cellular bud neck
Biological Context
Attribution
- Names
- David Ball
- Jean Peccoud
- Published
- PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
- Pubmed
- 22046265
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- phase contrast microscopy
- Parameters Imaged
- fluorescence emission
- elastic scattering of photons
- Source of Contrast
- distribution of a specific protein
- differences in amount of elastic light scattering
- Visualization Methods
- EGFP
- visualization of contiguous regions
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | 213.4nm |
| Y | 512px | 213.4nm |
| Z | 21 | .5μm |
| Time | 300 sec | 61 |
*CIL – Cell Image Library accession number. Please use this to reference an image.