A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Clb2. Clb 2 is a cyclin-dependent protein kinase regulator, involving in G2/M transition of mitotic cell cycle and positively regulating spindle pole body separation. These phase and GFP z-series images are part of an image group containing CIL 39556-39560. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.
- David Ball
- Jean Peccoud
- PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
GroupingThis image is representative of a group of similar images.
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- phase contrast microscopy
- Parameters Imaged
- fluorescence emission
- elastic scattering of photons
- Source of Contrast
- distribution of a specific protein
- differences in amount of elastic light scattering
- Visualization Methods
- visualization of contiguous regions
- Processing History
- unprocessed raw data
|Spatial Axis||Image Size||Pixel Size|