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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39503*  Cite 

Freeze fracture of Vorticella microstoma. This cell had been placed in a relaxing medium of saturated sodium pyrophosphate plus 0.01M EDTA (Stain Tech. 47:37, 1972) for 3 hours. A myoneme enclosed by ER membrane is fractured at the cell’s periphery. In this cell it appears that the ER wraps around the myoneme. The P-fracture face of the plasma membrane is studded with many IMPs of significant size in contrast to the E-face of the outer alveolar membrane which has few IMPs. Bits of the P-face of the inner alveolar membrane show many IMPs on that face also. The P-face of the cytosolic-touching membrane of the ER and the E-face of the myonemal-touching membrane of the ER are visible. Linear arrays of IMPs on the E-face next to the myoneme may be transmembrane proteins associated with the linkage complex. Mitochondria are the most prominent organelles in the cytosol and their tubular cristae sometimes show signs of decoration with F1F0 ATP synthases. large arrow, direction of shadowing of freeze fracture replica. TEM taken on 11/11/75 by R. Allen with JEM 100B operating at 80kV. Neg. 11,015X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Vorticella microstoma
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
endoplasmic reticulum
Biological Context
Biological Process
myofibril contraction
cytoplasm organization
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
shadowing and plating
recorded image
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 4420px 1.4nm
Y 5264px 1.4nm