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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39464*  Cite 
Description

Like other peritrichs the pellicle of Vorticella convallaria is sculpted into ridges and grooves that circle the cell perpendicular to the aboral/adoral axis of the cell body. Pellicular pores penetrate the pellicle as cylindrical indentations of the plasma membrane that end as clathrin-coated pits. A system of alveoli underlies the plasma membrane and rod-like densities occupy the cytosolic tips of the pellicular ridges. A fibrous epiplasm covers the inside of the alveoli. Distinct myonemal bands lie in the aboral to adoral direction to the inside of the epiplasm. These bands are associated with the endoplasmic reticulum by unique specializations of the ER. In this EM preparation the ER has artifactually segmented into vesicles caused by the fixation process. Mitochondria occupy the space between the bands. TEM taken on 4/15/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Vorticella convallaria
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component
pellicle
endoplasmic reticulum
mitochondrion
cell cortex
Biological Context
Biological Process
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39464
Archival Resource Key (ARK)
ark:/b7295/w9cil39464
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3726px 1.1nm
Y 4000px 1.1nm