V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhances the identification of loci where ER remains bound to the myonemes. These loci on the ER are called “linkage complexes." These complexes are unique and have not been reported outside the peritrichs. Macronucleus with nucleolus and cytopharynx at its distal end. TEM taken on 3/30/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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