Like other ciliates two types of nuclei are present. A germinative micronucleus and a RNA-producing macronucleus. Both have nuclear envelopes with pores and both have ribosomes attached to their outer membranes. Heterochromatin bodies are the only prominently stained entities in the micronucleus. A few microtubules are also present. TEM taken on 3/29/71 by R. Allen with Hitachi HU11A operating at 75kV. Mag. 13,750X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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