Licensing
Description
11 micrographs of views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 4 food vacuoles, 3 segments of the macronucleus, perioral cilia at the adoral pole that forms a wreath of cilia around the oral opening in the uncontracted cell, and the lower part of the food vacuole-forming cytopharynx. TEM taken on 4/5/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg 2,150X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Technical Details
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Vorticella convallaria
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Cambridge Culture Collection, Cambridge, England, # LB1690/1
- Cellular Component
- cell
- food vacuole
- macronucleus
- oral apparatus
- cytopharynx
Biological Context
Attribution
- Name
- Richard Allen (University of Hawaii)
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- recorded image
- film
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3840px | 2.3nm |
| Y | 4000px | 2.3nm |