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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39333*  Cite 
Description

HeLa cells expressing a kinetochore-specific protein were synchronized and released into S phase. At time intervals of 1,3,4,6,and 8 hr, cells were pulsed with BrdU, fixed and processed to label contromeres (red) and replicating DNA (green). Nuclear DNA was stained with DAPI (blue). Z-series image stacks were obtained using widefield microscopy, and the out-of-focus signal removed by constrained iterative deconvolution. Each column shows a single nucleus representative of the S-phase stages I - V. The top row (a-e) shows a merge of the red, green and blue signals, and the center row (f-j), the red and green signals only. The red and green signals were processed to reveal centromere-associated DNA replication which peaks in the latter half of S.

Technical Details

See Fig 2 in: RD Shelby et al. 2000 Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol 151:1113-1118.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
nucleus
condensed nuclear chromosome, centromeric region
Biological Context
Biological Process
DNA metabolic process
DNA replication
Attribution
Names
Richard Shelby
Karine Monier
Kevin Sullivan
Published
RD Shelby et al. 2000 Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol 151:1113-1118
Pubmed
11086012
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39333
Archival Resource Key (ARK)
ark:/b7295/w9cil39333
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 563px ——
Y 337px ——