The Cell: An Image Library - Image CIL:39321

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CIL:39321^*

Description

Quick-freeze deep-etch of granulo-fibrillar material at the crests of the ridges. This material is composed of thinner and shorter filaments that, like the striated bands, appear to bind to the membrane. The fracture plane also exposed the cell's exterior. TEM taken on 6/4/91 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex

Biological Context

Biological Process: cortical cytoskeleton organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4742px	0.77nm
Y	5484px	0.77nm

*CIL – Cell Image Library accession number. Please use this to reference an image.