The Cell: An Image Library - Image CIL:39319

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:39319^*

Description

The infraciliary lattice in this quick-freeze deep-etched image consists of a meshwork of branching fibers composed of many subfibers. Basal bodies and trichocyst tips extend through the openings of the mesh. Individual metal-coated fibrils of the mesh average 11 nm in diameter and are closely interlaced and cross-linked to other fibrils. TEM taken on 5/27/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; mitochondria; trichocyst; cilium; cytoplasm

Biological Context

Biological Process: cortical cytoskeleton organization; cytoplasm organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5498px	1.4nm
Y	6001px	1.4nm

*CIL – Cell Image Library accession number. Please use this to reference an image.