The Cell: An Image Library - Image CIL:39314

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CIL:39314^*

Description

In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: plasma membrane

Biological Context

Biological Process: plasma membrane organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4892px	0.8nm
Y	5550px	0.8nm

*CIL – Cell Image Library accession number. Please use this to reference an image.