The Cell: An Image Library - Image CIL:39313

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CIL:39313^*

Description

Freeze fracture image of the surface ridges at the anterior end of the cell where kineties to the right and left sides come together at an angle at the anterior suture tip. In this area are numerous plates of IMPs in the P-face of the plasma membrane. Rings and rosette IMPs at the trichocyst exocytic sites are also seen. TEM taken on 10/2/80 by R. Allen with HU 11A operating at 75kV. Neg. 10,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: pellicle; plasma membrane; trichocyst

Biological Context

Biological Process: plasma membrane organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5235px	1.5nm
Y	4578px	1.5nm

*CIL – Cell Image Library accession number. Please use this to reference an image.