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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39303*  Cite 

High magnification serial block face scanning electron microscopy image series of a cell-cell contact region between four cells in the interior of the multilayered region of an FGF2 (fibroblast growth factor 2) induced mammary organoid in 3D Matrigel culture. Membrane protrusions are extensive, vary continuously from linear to branched, and collectively account for extensive contact area between the cells. Individual images are at 50 nm intervals. The field of view is 5.6 microns wide. This image is part of an image series CIL:39301-39305.

Technical Details

Organoids embedded in resin were mounted onto an aluminum pin with a cyanoacrylate adhesive. The pin, which takes the place of a normal SEM stub, was loaded into a sample holder for the Gatan 3View (Gatan Inc., Pleasanton, Ca). Serial block face scanning electron microscopy was carried out as previously described (Denk and Horstmann, 2004). Data was collected using an FEI Quanta 600 FEG SEM; serial images were 4k x 4k and acquired at 5 kV; z-dimension slices of 50 nm. 3D volume representations of the data were prepared using CHIMERA(Pettersen et al., 2004). CHIMERA was used to perform manual segmentation of membranes and to display the 3-dimensional volumes.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
epithelial cell
Cellular Component
cell-cell contact zone
Biological Context
Biological Process
cell-cell junction organization
Mouse Gross Anatomy
mammary organoid
Andrew Ewald
J Cell Sci. 2012 Feb 17. [Epub ahead of print]
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
serial block face SEM (SBFSEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
glutaraldehyde fixed tissue
microwave processing
tissue in epoxy resin embedment
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
Z —— 50nm