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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39255^* Cite

Description

Mouse myoblasts stably transfected with GFP-PCNA to label replication foci and the 3D distribution of the GFP signal recorded over time using a confocal microscope. The time series illustrates the dynamic changes in relative strength of the GFP signal in individual replication factories.

Technical Details

Living C2C12 cells stably expressing GFP-PCNA were observed with a Leica TCS confocal microscope and z-series collected at 0.5 micrometer intervals over a 90 min period. See: Fig 6B in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: myoblast
Cell Line: C2C12
Cellular Component: nuclear chromatin; nuclear DNA

Biological Context

Biological Process: DNA replication; DNA metabolic process

Attribution

Names: Heinrich Leomhardt; Hans-Peter Rahn; Peter Weinziert; Anje Sporbert; Thomas Cremer; Daniele Zink; M Cristina Cordoso
Published: H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
Pubmed: 10769021

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39255
Archival Resource Key (ARK): ark:/b7295/w9cil39255

Grouping This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT); charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	999px	——
Y	219px	——