Mouse myoblast cells were engineered to express GFP-PCNA (proliferating cell nuclear antigen) as a marker for replication factories, and also immunostained for DNA ligase. DNA was localized using Hoechst staining. The image shows two different typical S-phase patterns displaying GFP-PCNA (left), DNA ligase (center) and DNA (right). DNA ligase co-localizes with PCNA at replication foci.
C2C12 cells stably expressing GFP-PCNA were fixed,stained for Dnmt1 and DNA, and optical sections acquired with a Leica TCS confocal microscope. See: Fig 2A in H Leonhardt et al. 2000 Dynamics of DNA replication factories in living cells. J Cell Biol 149:271-279
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