The Cell: An Image Library - Image CIL:39242

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CIL:39242^*

Description

The macronucleus, one micronucleus, and the cytoplasm of Conchophthirus. The negative magnification is 6,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources

NCBI Organism Classification: uncultured scuticociliate; Conchophthirus curtus
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: macronucleus; micronucleus; cytoplasm

Biological Context

Biological Process: macronucleus organization; micronucleus organization; cytoplasm organization

Attribution

Name: Gregory Antipa (San Francisco State University)
Link: http://online.sfsu.edu/~antipa/

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3865px	1.5nm
Y	4000px	1.5nm

*CIL – Cell Image Library accession number. Please use this to reference an image.