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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39201*  Cite 
Description

A bundle of microtubules that arises from a basal body adjacent to the cytoproct comes to lie along a spent DV-IV. The assumption is that movement generated by molecular motors propels the spent vacuole toward the cytoproct. TEM taken on 6/1/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Published in J. Cell Sci. 14:611-631, 1974. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytoproct
microtubule associated complex
vesicle membrane
Biological Context
Biological Process
digestive system process
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Sci. 1974 May;14(3):611-31.
Published
4364579
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39201
Archival Resource Key (ARK)
ark:/b7295/w9cil39201
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4500px 1.2nm
Y 5148px 1.2nm