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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39177*  Cite 
Description

The collapsed cytopharyngeal membrane extends from the open cytopharynx into the cytosol. This membrane is associated with many docked acidosomes, vesicles that contain V-type proton translocating ATPases and cause the acidification of the phagosome after they fuse with the phagosome. TEM taken on 11/4/68 by R. Allen with Philips 300 operating at 60kV. Neg.16,000X. Published in J. Cell Biol. 64:497-503, 1975. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
vacuolar proton-transporting V-type ATPase, V0 domain
cytopharynx
Biological Context
Biological Process
phagocytosis
digestive system process
digestion
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Biol. 1975 Feb;64(2):497-503.
Published
1117032
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39177
Archival Resource Key (ARK)
ark:/b7295/w9cil39177
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4832px 0.94nm
Y 6317px 0.94nm