Licensing
Description
Detail of the junction between the ribbed wall and the right side of the cytopharynx area. The ribbed wall is covered by alveoli and the ribs are produced by small narrow ridges similar to the larger ridges covering the whole cell body. Two arrays of microtubules are present in this zone, clumps of hexagonally packed bundles lying under the ridges and rows of microtubules that lie under the single cytopharyngeal membrane. There is some suggestion that these rows of microtubules may associate together to form the bundles. Tips of cilia in cross section give 4 views of how the tips of cilia end, the 9-doublet first become 9 singlets and then these are lost one by one leaving only the two central singlets at the tip of a cilium. Sections of the cytostomal cord are also present under the rib wall. This is the cord that wraps around the cytopharynx. TEM taken on 7/27/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 23,000X.
Technical Details
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Paramecium caudatum
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cellular Component
- oral apparatus
Biological Context
- Biological Process
- oral apparatus organization
Attribution
- Name
- Richard Allen (University of Hawaii)
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- uranyl salt
- lead salt
- osmium tetroxide
- Processing History
- recorded image
- film
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3256px | 0.87nm |
| Y | 3767px | 0.87nm |