Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39128^* Cite

Description

Cytopharyngeal basket in tangential section is composed of a complex of microtubular rods. The lumen of the basket contains many vesicles called cytopharyngeal vesicles. They may provide membrane for the nascent food vacuole, lysosomal and/or lytic enzymes, or all of these. Another role may be to provide vacuolar type ATPases (proton pumps) to the nascent food vacuole. TEM taken on 3/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: oral apparatus; microtubule associated complex; symbiont-containing vacuole

Biological Context

Biological Process: oral apparatus organization; microtubule bundle formation; bacterial symbiont interaction

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39128
Archival Resource Key (ARK): ark:/b7295/w9cil39128

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4000px	1.4nm
Y	2948px	1.4nm