Wheat seeds were germinated and grown for 6 days in the dark, greened at high light intensity for 4 days, thylakoids prepared, plunge-frozen, and freeze-fracture replicas obtained and examined by transmission electron microscopy. This region from a wild type plant shows the characteristic particle size and distribution found on the EF and PF membrane faces, with the different distributions between stacked (PFs and EFs) and unstacked (PFu, EFu)thylakoid regions. This set of grouped images, which also shows thin sections, includes images of the chlorophyll b-deficient CD3 mutant.
Thylakoid preparations were equilibrated with 35% glycerol, plunge frozen in liquid Freon 12 cooled in liquid N2 and fractured and replicated in a Balzers BA 360 freeze etch device. Replicas were examined in a JEOL 100C at 80KV and images recorded on film. See Fig 8 in K D Allen et al. 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919.