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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39062*  Cite 

This light micrograph shows the outside edge of two seminiferous tubules of a mouse testis. This section is a 1um thick transverse section, stained with toluidine blue to highlight the cells, with the nucleus staining a darker blue. The dark blue line separating the two seminiferous tubules consists mostly of myoid (muscle) tissue. The majority of cells seen in this image (arranged in layers) are germ cells, which, by repeated cell division, eventually produce spermatozoa. Chromosomes are visible in most of the nuclei of the cells. The cell with a deeply-stained nucleus and even darker nucleolus is a Sertoli cell ('nurse cell') that has fine cytoplasmic extensions branching between the other cells to nurture the developing germ cells.

Technical Details

B0007567 Male germ cells. Wellcome Images available under the following creative commons usage

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
male germ cell
Sertoli cell
Cellular Component
nuclear chromosome
Biological Context
Biological Process
seminiferous tubule development
Mouse Gross Anatomy
seminiferous tubule
myoid tissue
Spike Walker
Wellcome Images
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
modes of light microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
toluidine blue
Processing History
unprocessed raw data
Sample Preparation
chemically fixed tissue
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 800px ——
Y 500px ——