Leaves from the arc5 plastid division mutant of Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ2-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. The protein is localized at the site of division which occurs by constricting at the chloroplast mid-point. The upper image shows the fluorescence signal, the lower panel, the corresponding DIC image.
Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119.
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