Alternate header for print version

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38828*  Cite 

High resolution overview of Coleps hirtus cell seen in this median transverse section. This barrel-shaped cell has a few rows of cilia and the cell is covered with calcified polysaccharide platelets that are housed within the alveoli. In this TEM the platelets are absent as they dissolved during preparation for electron microscopy. The macronucleus, a food vacuole, and a large empty vacuole is evident. Typical ciliate cytoplasm is present. TEM taken on 5/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,120X.

Technical Details

The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information is available at (

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
plasma membrane
alveolar membranes
contractile vacuole
kinetal rows of cilia
Biological Context
Biological Process
ciliary or flagellar motility
detection of calcium ion
Molecular Function
calcium deposition
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
Print from negative scanned for Photoshop.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 3590px 1.6nm
Y 4000px 1.6nm