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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38805*  Cite 
Description

Confocal micrograph showing the expression of different fluorescent proteins in the stem of a thale cress seedling (Arabidopsis thaliana) used to study in vivo gene expression. Arabidopsis was the first plant to have its entire genome sequenced and is an important model for studying plant biology. These genetically modified plants have fluorescent proteins associated with different gene promoters. In these plants, when a promoter switches a gene on, a corresponding fluorescent protein is produced, allowing expression of these genes to be viewed. In this image green fluorescence (GFP) shows the expression of the gene under investigation and the red fluorescence corresponds to the expression of a known gene and thus acts as a reference. Cells that are yellow express both genes. A third fluorescent protein is attached to the plasma membrane to show the outline of each cell. Small red cells that don't have the gene active and do not express GFP are precursors of stomata.

Technical Details

B0007807 Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://creativecommons.org/licenses/by-nc-nd/2.0/uk/

Biological Sources
NCBI Organism Classification
Arabidopsis thaliana
Cellular Component
plasma membrane
nucleus
Biological Context
Biological Process
gene expression
Attribution
Names
Fernan Federici
Jim Haseloff
Link
Wellcome Images
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38805
Archival Resource Key (ARK)
ark:/b7295/w9cil38805
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific nucleic acid sequence
Visualization Methods
EGFP
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 470px ——
Y 576px ——