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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38711*  Cite 
Description

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Under the isolation conditions used, thylakoid membranes from the ac-5 mutant are unstacked. See Fig 13 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM.

Biological Sources
NCBI Organism Classification
Chlamydomonas reinhardtii
Cell Line
ac-5
ac-31
Cellular Component
chloroplast
plastid
plastid thylakoid membrane
Biological Context
Biological Process
photosynthesis
organelle organization
Attribution
Names
Ursula W. Goodenough
L. Andrew Staehelin
Published
UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed
4396088
Link
Journal of Cell Biology
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38711
Archival Resource Key (ARK)
ark:/b7295/w9cil38711
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
uranyl salt
lead salt
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1332px ——
Y 1374px ——