Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. These thylakoid membranes from this mixotropically grown ac-5 mutant show the particle size and distribution typical of unstacked membranes. See Fig 16 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.
Membranes were prepared, cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619.
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