The series of three vertical columns shows various aspects of mitotic anaphase in a live Barbulanympha (protist). The left column shows the birefringent spindle imaged by polarization microscopy; the center column contains a merge of the polarizing image with differential interference (DIC) microscopy and reveals the chromosomes in a plane near the upper surface of the spindle, and the right hand column, also using merged polarization and DIC, shows the relationship between the spindle and chromosomes in a median optical section of the spindle.
Inoue and Ritter used high-quality sequential coronal polarization (left) and differential interfrence contrast (DIC) microscopy. In the middle column, the focus on the upper surface shows the parting seam in the nuclear envelope (between arrow pairs). The right column shows the median optical section. See: Inoué S, Ritter H Jr. (1978). Mitosis in Barbulanympha. II. Dynamics of a two-stage anaphase, nuclear morphogenesis, and cytokinesis. J Cell Biol. 77:655-84.
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