This image combines total internal reflection microscopy (TIRF) of mCerulean-actin (blue) with photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green). Vinculin and paxillin are organized into focal adhesions at the ends of actin bundles. A higher magnification of several adhesion complexes (CIL 38651) clearly shows that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.
|Spatial Axis||Image Size||Pixel Size|