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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38652*  Cite 
Description

This image combines total internal reflection microscopy (TIRF) of mCerulean-actin (blue) with photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green). Vinculin and paxillin are organized into focal adhesions at the ends of actin bundles. A higher magnification of several adhesion complexes (CIL 38651) clearly shows that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
foreskin fibroblast
Cellular Component
focal adhesion
actin cytoskeleton
vinculin
paxillin
Biological Context
Biological Process
molecular organization
Attribution
Names
Hari Shroff
Catherine G Galbraith
James A. Galbraith
Helen White
Jennifer Gillette
Scott Olenych
Michael W. Davidson
Eric Betzig
Published
Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Pubmed
18077327
Link
article
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38652
Archival Resource Key (ARK)
ark:/b7295/w9cil38652
Grouping This image is part of a group.
Imaging
Image Type
portrayed image
recorded image
Image Mode
TIRF (total internal reflection)
PALM (photoactivation localization microscopy)
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
CeruleanFP
tdEos
Dronpa
Processing History
probability density map
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 2124px ——
Y 1758px ——