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Description
Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green). This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated. The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13.
Biological Sources
- NCBI Organism Classification
- Homo sapiens
- (human)
- Cell Type
- fibroblast
- Cell Line
- foreskin fibroblast
- Cellular Component
- focal adhesion
- actin cytoskeleton
- alpha-actinin
- vinculin
Biological Context
- Biological Process
- molecular organization of focal adhesion components
- focal adhesion assembly
Attribution
- Names
- Hari Shroff
- Catherine G Galbraith
- James A. Galbraith
- Helen White
- Jennifer Gillette
- Scott Olenych
- Michael W. Davidson
- Eric Betzig
- Published
- Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
- Link
- article
- Pubmed
- 18077327
Grouping
This image is part of a group.Imaging
- Image Type
- portrayed image
- Imaging Mode
- PALM (photoactivation localization microscopy)
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- Visualization Methods
- Dronpa
- tdEos
- Processing History
- probability density map
Sample Preparation
- Methods
- formaldehyde fixed tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 5787px | —— |
| Y | 4865px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.