Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. The embryo was treated with the drug cytochalasin D that disrupts the actin cytoskeleton. Following drug treatment, the boundaries of PAR-2 and PAR-6 shift to the posterior as PAR-2 appears to be removed from the cortex and sequestered in cytoplasmic aggegates.
C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Imaged using an Olympus IX71 equipped with a Yokogawa spinning-disk head using a 60X-1.35 oil UPlanSApo objective, 488- and 561-nm lasers (DPSS) and an Andor iXon camera with ImageIQ. Images captured at embryo midplane at 2-2.5 second intervals. Time-lapse images correspond to Fig 4I in J Cell Biol. 2011. 193(3): 583-594.
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