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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37412*  Cite 

Zebrafish Embryo, from tailbud to 16-hour stage, stained with 100 micromolar Bodipy-Ceramide. Bodipy-Ceramide permeates the enveloping layer epithelium of the embryo, accumulating in the interstitial spaces of the segmentation cavity. The sequence begins at the level of the hypoblast. Chorda mesoderm cells intercalate in a mediolateral fasion. This cellular convergence causes the chorda mesoderm to extend in an axial direction. During cell intercalation, the notochord develops a transient bilateral symmetry. As the sequence progresses, the notochord is depressed ventrally by mediolateral convergence of cells in the neural plate (this begins neural keel formation). The floorplate comes into view. Cells at the level of the floorplate along the embryonic midline begin to round up in preparation for mitosis. At one point at least four cells are undergoing synchronous division. The daughter cells of these divisions segregate to either side of the embryonic midline. The embryo undergoes a torsional rotation. The rotation brings into view the edge of the neural keel at the level of the hindbrain. The ridges along the edge of the neural keel delineate neuromeres of the hindbrain. The sequence ends with a view of the otic placode. The time-lapse sequence compresses approximately 11 hours of development. This video made available by Mark Cooper thru Zebrafish - The Living Laboratory. The videos in this group can also be found in the Developmental Landmarks page of the Fishscope web site.

Biological Sources
NCBI Organism Classification
Danio rerio
Cell Type
epithelial cell
chorda mesoderm
Cell Line
Embryonic cell
Cellular Component
interstitial space
Biological Context
Biological Process
anatomical structure morphogenesis
mediolateral convergence
cell division
Zebrafish Anatomy
neural plate
neural keel
floor plate
otic placode
Mark Cooper
Zebrafish - The Living Laboratory
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
visualization of contiguous regions
bodipy ceramide
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue