Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37402*  Cite 

Four cell stage zebrafish embryo, stained with 100 micromolar Bodipy 505/515. In the upper portion of the image, the yolk cell contains large spherical yolk platelets. The blastomeres undergoing meroblastic cleavage in the lower portion of the image are joined by cytoplasmic bridges to the yolk cell. Cell nuclei are not stained by Bodipy 505/515, and thus appear as dark circles. Bodipy 505/515 partitions into yolk platelets which appear as white spheres and particles distributed through the cytoplasm of the blastomeres. Yolk platelets within the cytoplasm of the yolk cell are also intensely stained. During every prophase there is a major onset of motility of the yolk platelets. The platelets are pulled into tubular forms, sometimes fragmenting. During telophase and interphase, the platelets re-coalesce into spherical bodies. Larger yolk platelets tend to be localized in the peripheral cytoplasm of cleaving blastomeres. Also notice the periodic fragmentation of the yolk mass within the yolk cell. The sequence continues through 16-cell stage. This video made available by Mark Cooper thru Zebrafish - The Living Laboratory. The videos in this group can also be found in the Developmental Landmarks page of the Fishscope web site.

Technical Details

Frames were taken at 20 second intervals using a 20X lens with 0.75 N.A.

Biological Sources
NCBI Organism Classification
Danio rerio
Cell Type
yolk cell
Cell Line
Embryonic cell
Cellular Component
yolk platelets
Biological Context
Biological Process
anatomical structure morphogenesis
Mark Cooper
Zebrafish - The Living Laboratory
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 320px ——
Y 240px ——