The video shows a cytoplast that is adhering to a sub-stratum in its central portion. It is also connected by a retraction fiber to its central cell. The cytoplast is engaging in vigorous protrusive activity around its periphery. The small lamellipodia is undergoing ruffling--extensions, folding back, and re-entry of the the protrusion into the main process of the cell. This demonstrates that an anucleate fragment of a cell has the capacity to nucleate actin, engage in ruffling, and retrograde flows. CIL 37336 and 37337 show that these fragments have the capacity to undergo directional locomotion. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.
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