The Cell: An Image Library - Image CIL:37299

Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

Comments:

Add to Photobox

CIL:37299^*

Description

Transmission electron micrograph of rough microsomes obtained by fraction of guinea pig pancreas. Cell fractionation confirmed that newly synthesized acinar cell secretory proteins moved from the rough endoplasmic reticulum to the periphery of the Golgi in the lumen of a smooth-surfaced membrane bounded compartment. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Additional technical details regarding cell fractionation, specimen processing, and electron microscopy can be found in: Jamieson, J.D., and Palade, G.E. J. Cell Biol. 34:577-596, 1967. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x10,000

Biological Sources

NCBI Organism Classification: Cavia porcellus; (Guinea Pig)
Cell Type: pancreatic cell
Cellular Component: rough microsome

Biological Context

Biological Process: protein secretion

Attribution

Name: James D. Jamieson
Link: George E. Palade EM Slide COllection
Date: 02/02/1967

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: lead salt; uranyl salt
Processing History: scanned negative

Sample Preparation

Methods: osmium tetroxide fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	6000px	——
Y	4530px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.